The following example shows how you can alternate item colors in a Flex Tree control by setting the alternatingItemColors style to an array of colors

<?xml version="1.0" encoding="utf-8"?>
<!-- http://blog.flexexamples.com/2007/11/27/alternating-row-colors-in-a-flex-tree-control-using-the-alternatingitemcolors-style/ -->
<mx:Application xmlns:mx="http://www.adobe.com/2006/mxml"
        layout="vertical"
        verticalAlign="middle"
        backgroundColor="white">

    <mx:XMLListCollection id="xmlListColl">
        <mx:source>
            <mx:XMLList>
                <node label="One">
                    <node label="One.1" />
                    <node label="One.2" />
                    <node label="One.3" />
                </node>
                <node label="Two">
                    <node label="Two.1" />
                    <node label="Two.2" />
                    <node label="Two.3" />
                </node>
            </mx:XMLList>
        </mx:source>
    </mx:XMLListCollection>

    <mx:Tree id="tree"
            dataProvider="{xmlListColl}"
            labelField="@label"
            alternatingItemColors="[#DFDFDF,#EEEEEE]"
            rowCount="4"
            width="300" />

</mx:Application>